*Speaker*

Dr. Helmut Schlumprecht

Statistik-Service Schlumprecht

**Date**

Course 1: 7 – 9 November, 2018

Course 2: 21 – 23 November, 2018

**Location**

Freiburg

Berlin

**Introduction into descriptive statistics**

- Overview: Measures of central tendency and spread, i.e.

- Arithmet. mean, range, standard deviati on, standard error, confidence interval for the mean
- Median, interquartile range, confidence interval for the median

- Explanation of the requirements for statistical parameters
- Which measure for what purpose?

**Introduction into using the program**

- Data organisati on in GraphPad Prism
- Computing descriptive statistics in GraphPad Prism
- Differences to other statistical programs

**Creating simple graphs in GraphPad Prism**

- Data organisation in GraphPad Prism
- Computing descriptive statistics in GraphPad Prism
- Differences to other statistical programs

**Introduction in inference statistics:**

Overview: Theory and diversity of statistical tests

- Comparing two groups

A: Checking the requirements

- Visual inspection of scatterplots and histograms
- Tests of Normality and of outliers
- Tests of homogeneity of variances

B: Comparing paired and unpaired groups

- t-Test
- Mann-Whitney U-Test, Wilcoxon – matched pairs signed rank test

2. Checking three or more groups – Overview

- Oneway-ANOVA – analysis of variance and post hoc-Tests
- Kruskal-Wallis –Test (Oneway-ANOVA of ranks and post hoc-Tests)

Which Test for what purpose? Which requirements?

**Using GraphPad-Prism**

- Comparing two groups, tutorial data sets from GraphPad Prism
- t-Test
- Mann-Whitney U-Test
- Wilcoxon – matched pairs signed rank test

**Graphs for comparisons of two groups**

- Boxplot and arrows for significance and labeling
- Editing of simple graphs
- Using GraphPad-Prism

**Questions**

**Comparing three or more groups: Analysis of variance (ANOVA)**

- Oneway-ANOVA and post hoc-Tests
- Kruskal-Wallis–Test (Oneway-ANOVA for ranks and post hoc tests)
- Repeated measure – design

**Introduction and data organisation in GraphPad Prism for Oneway-ANOVA**

**Linear regression – analysing relations between numeric x and y-values and their graphing**

- Importance
- Procedures
- Graphs

**Criteria for good scientific graphs**

- Diversity of graph types – advantages and disadvantages
- Requirements and use
- Graphs for small and large data sets

**Editing of graphs**

- Procedures
- Graphs tutorial and graph Portfolio of GraphPad Prism

**Introduction into Non linear regression (Enzyme kinetics, Michelis-Mentenkinetics, ELISA etc.)**

- Theory and using the program

**Non linear regression – further topics and using the program**

- Theory and using the program

**Questions**

**Speeding up the workflow (Data analysis and graphs for test series – from prototype to serial production)**

**Introduction into the program and data organisation in GraphPad Prism for serial production**

**GraphPad Tutorial and Internet help**

- Examples and tutorial data sets for analyses and graphs
- Don’t panic – How to use the help pages of GraphPad Prism

**Analysis of frequency data (counts; chisquare tests etc.)**

- Importance
- Procedures
- Graphs